Instrumental Methods of Analysis

Syllabus

 Module 01                                                                                                                        

UV Visible Spectroscopy

• Electronic transitions, chromophores, auxochromes, spectral shifts, solvent effect on absorption spectra, Beer and Lambert’s law, Derivation and deviations.
• Instrumentation - Sources of radiation, wavelength selectors, sample cells, detectors-Photo tube, Photomultiplier tube, Photo voltaic cell, Silicon Photodiode.
• Applications - Spectrophotometric titrations, Single component and multi component analysis.

Fluorimetry

• Theory, Concepts of singlet, doublet and triplet electronic states, internal and external conversions, factors affecting fluorescence, quenching, instrumentation and applications. 

Module 02                                                                                                                         IR spectroscopy

Introduction, fundamental modes of vibrations in poly atomic molecules, samplehandling, factors affecting vibrations.

Instrumentation - Sources of radiation, wavelength selectors, detectors - Golay cell, Bolometer, Thermocouple, Thermister, Pyroelectric detector and applications.

Flame Photometry

• Principle, interferences, instrumentation and applications.

Atomic Absorption Spectroscopy

Principle, interferences, instrumentation and applications.

Nepheloturbidometry

Principle, instrumentation and applications.

Module 03                                                                                                                       Introduction to chromatography

Adsorption and partition column chromatography

• Methodology, advantages, disadvantages and applications.

Thin layer chromatography

• Introduction, Principle, Methodology, R f va lu es, advantages, disadvantages and

applications.

Paper Chromatography

• Introduction, methodology, development techniques, advantages, disadvantages and applications.

Electrophoresis

•  Introduction, factors affecting electrophoretic mobility, Techniques of paper, gel,

capillary electrophoresis, applications.

Module 04                                                                                                                        

Gas Chromatography

• Introduction, theory, instrumentation, derivatization, temperature programming,

advantages, disadvantages and applications.

High Performance Liquid Chromatography (HPLC)

• Introduction, theory, instrumentation, advantages and applications.

Module 05                                                                                                                          Ion exchange chromatography

• Introduction, classification, ion exchange resins, properties, mechanism of ion
• exchange process, factors affecting ion exchange, methodology and applications.

Gel chromatography

• Introduction, theory, instrumentation and applications.

Affinity chromatography

• Introduction, theory, instrumentation and applications.