Advanced Instrumentation Techniques B.Pharm 8th Semester

                                                                     Syllabus

                      Advanced Instrumentation Techniques

                                                        (Course Code: BP811ET)

Module 01                                                                                                                                                                                       10 Hours

Nuclear Magnetic Resonance Spectroscopy

·         Principles of H-NMR and C-NMR, Chemical Shift, Factors Affecting Chemical Shift, Coupling Constant, Spin - Spin Coupling, Relaxation, Instrumentation and Applications

Mass Spectrometry

·         Principles, Fragmentation, Ionization Techniques – Electron Impact, Chemical Ionization, MALDI, FAB, Analyzers-Time of Flight and Quadrupole, Instrumentation, Applications.

Module 02                                                                                                                                                                                       10 Hours

Thermal Methods of Analysis

·         Principles, Instrumentation and Applications of Thermogravimetric Analysis (TGA), Differential Thermal Analysis (DTA), Differential Scanning Calorimetry (DSC)

X-Ray Diffraction Methods

·         Origin of X-rays, basic Aspects of Crystals, X- ray Crystallography, Rotating Crystal Technique, Single Crystal Diffraction, Powder Diffraction, Structural Elucidation and Applications.

Module 03                                                                                                                                                                                       10 Hours

Calibration and Validation as Per ICH and USFDA Guidelines

Calibration of Following Instruments

·         Electronic Balance, UV-Visible Spectrophotometer, IR Spectrophotometer, Fluorimeter,

Flame Photometer, HPLC and GC.

Module 04                                                                                                                                                                                       08 Hours

Radioimmuno Assay

·         Importance, Various Components, Principle, Different Methods, Limitation and Applications of Radioimmuno Assay

Extraction Techniques

·         General Principle and Procedure Involved in the Solid Phase Extraction and Liquidliquid Extraction.

Module 05                                                                                                                                                                                       07 Hours

Hyphenated Techniques

·         LC-MS/MS

·         GC-MS/MS

·         HPTLC-MS